Chemistry
Area of Doctoral Study: Chemistry
Undergraduate Institute: Shahjalal University of Science and Technology
Graduate Institute: Shahjalal University of Science and Technology
Graduate Institute: Mississippi State University
Research Advisor: Deepak Koirala, PhD.
Description of Research
My current research project focuses on determining high-resolution crystal structures of viral RNA domains associated with the cap-independent translation initiation in positive (+)-sense single-stranded (ss) RNA viruses and understanding their functional roles using synthetic antibodies. My projects involve the synthesis and purification of viral RNA constructs as well as the expression and purification of the antibodies – especially the Fab fragments. It also involves characterization of Fab-RNA binding using various molecular biology, biochemical and biophysical techniques and setting up the crystallization trials. Once Fab-RNA crystals are obtained, the diffraction data are collected at Argonne National Lab, and the suitable datasets are used to solve the high-resolution crystal structures.